Regulatory

Part:BBa_K3841008:Design

Designed by: Emil Ørsted Christensen   Group: iGEM21_DTU-Denmark   (2021-08-20)


Methanol regulated promoter AOX1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We retrieved this sequence from genebank and intent to test how effective it is compared to the original AOX1 promoter from https://parts.igem.org/Part:BBa_I764001. Additionally, we removed any BsaI and BsmBI sites within the coding sequence.


Source

Komagataella pastoris strain ATCC 28485 chromosome 4 sequence GenBank: CP014587.1 1..1577441 /organism="Komagataella pastoris" /mol_type="genomic DNA" /strain="ATCC 28485" /type_material="type material of Zygosaccharomyces pastoris" /db_xref="taxon:4922" /chromosome="4" /country="USA" /collection_date="2014"

References