Regulatory
Part:BBa_K3841008:Design
Designed by: Emil Ørsted Christensen Group: iGEM21_DTU-Denmark (2021-08-20)
Methanol regulated promoter AOX1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We retrieved this sequence from genebank and intent to test how effective it is compared to the original AOX1 promoter from https://parts.igem.org/Part:BBa_I764001. Additionally, we removed any BsaI and BsmBI sites within the coding sequence.
Source
Komagataella pastoris strain ATCC 28485 chromosome 4 sequence GenBank: CP014587.1 1..1577441 /organism="Komagataella pastoris" /mol_type="genomic DNA" /strain="ATCC 28485" /type_material="type material of Zygosaccharomyces pastoris" /db_xref="taxon:4922" /chromosome="4" /country="USA" /collection_date="2014"